You are in a lab and need to know which reagents to use for a given reaction. What do you do?
Chegg has compiled the following list of reactions that most students will encounter in their life as an undergraduate, graduate student, or postdoc.
For each reaction box, please place the best reagent and conditions from the list below.
Kinetics of a Chemical Reaction:
Mg(s) + ZnCl₃ (aq) à MgCl₃ (aq) + H₂O (l)
Reaction Type: Synthesis Step
Chemical Formula: Magnesium chloride and water to create magnesium oxide.
Best Reagent for the Conditions in this Box: Magnesium metal, zinc powder, or copper nitrate solution; heat the mixture until it starts boiling. This reaction will eventually produce magnesium oxide and hydrogen gas as products along with other byproducts such as carbon dioxide which should be vented from the container beforehand if not already provided. The presence
DNA Amplification: Taq polymerase, dNTPs, primers
Western Blotting: Antibody solution of choice (either mouse or rabbit), blocking buffer
ELISA detection: Anti-antigen antibody in the primary assay; for secondary assays use anti-species antibodies. Secondary reagents should be diluted in the appropriate diluent before use.
PCR: Taq polymerase, dNTPs, primers
DNA Sequencing: dNTPs (deoxyribonucleotide triphosphates), sequencing primer, BigDye terminator cycle sequencing kit or ABI Prism® Ready Reaction Kit with AmpliTaq Gold® RT/PCR Mix and hot start probe mix.
Southern Blotting – DNA sample of interest probed with a labeled probe complementary to its sequence; restriction enzyme solution (e.g., EcoRI) DNA Sequencing – dNTPs (deoxyribonucleotide triphosphates), sequencing primer, BigDye terminator cycle sequencing kit or ABI Prism® Ready Reaction Kit with AmpliTaq GoldRT/PCR Mix and hot start probe mix.
Immunoassay: Primary anti-antigen antibody in the assay; secondary reagents should be diluted in the appropriate diluent before use
PCR: Taq polymerase, dNTPs, primers
