Rantdriven
  • Home
  • Blog
  • Gaming
  • Fashion
  • Business
  • Animations
  • More
    • Write for us
    • About Us
    • Contact Us
    • Editorial Policy
    • Policy of cookies
    • Privacy Policy
    • Terms and Conditions
Rantdriven
Rantdriven
  • Home
  • Blog
  • Gaming
  • Fashion
  • Business
  • Animations
  • More
    • Write for us
    • About Us
    • Contact Us
    • Editorial Policy
    • Policy of cookies
    • Privacy Policy
    • Terms and Conditions
Blog

BEST Reagents for the Following Conditions:

by Linnea D June 21, 2021
by Linnea D June 21, 2021 0 comment 105 views

You are in a lab and need to know which reagents to use for a given reaction. What do you do?

Chegg has compiled the following list of reactions that most students will encounter in their life as an undergraduate, graduate student, or postdoc.

For each reaction box, please place the best reagent and conditions from the list below.

Kinetics of a Chemical Reaction:

Mg(s) + ZnCl₃ (aq) à MgCl₃ (aq) + H₂O (l)

Reaction Type: Synthesis Step

Chemical Formula: Magnesium chloride and water to create magnesium oxide.

Best Reagent for the Conditions in this Box: Magnesium metal, zinc powder, or copper nitrate solution; heat the mixture until it starts boiling. This reaction will eventually produce magnesium oxide and hydrogen gas as products along with other byproducts such as carbon dioxide which should be vented from the container beforehand if not already provided. The presence

DNA Amplification: Taq polymerase, dNTPs, primers

Western Blotting: Antibody solution of choice (either mouse or rabbit), blocking buffer

ELISA detection: Anti-antigen antibody in the primary assay; for secondary assays use anti-species antibodies. Secondary reagents should be diluted in the appropriate diluent before use.

PCR: Taq polymerase, dNTPs, primers

DNA Sequencing: dNTPs (deoxyribonucleotide triphosphates), sequencing primer, BigDye terminator cycle sequencing kit or ABI Prism® Ready Reaction Kit with AmpliTaq Gold® RT/PCR Mix and hot start probe mix.

Southern Blotting – DNA sample of interest probed with a labeled probe complementary to its sequence; restriction enzyme solution (e.g., EcoRI) DNA Sequencing – dNTPs (deoxyribonucleotide triphosphates), sequencing primer, BigDye terminator cycle sequencing kit or ABI Prism® Ready Reaction Kit with AmpliTaq GoldRT/PCR Mix and hot start probe mix.

Immunoassay: Primary anti-antigen antibody in the assay; secondary reagents should be diluted in the appropriate diluent before use

PCR: Taq polymerase, dNTPs, primers

Share
0
FacebookTwitterPinterestEmail
Linnea D

I am a blogger who loves to write and read blogs. I specialize in all types of posts, including social media support.

Leave a Comment Cancel Reply

Save my name, email, and website in this browser for the next time I comment.

Popular Posts

  • powered by emeeting

    March 1, 2022
  • powered by emeeting

    March 1, 2022
  • 3

    Stand Out in the Crowd: Open Shoulder Top

    June 19, 2021
  • powered by emeeting

    March 1, 2022
  • 5

    Reviews and Ratings of Angie’s List in San Diego

    June 22, 2021
  • Facebook
  • Twitter
  • Instagram
  • Pinterest
  • Home
  • About Us
  • Contact Us
  • Terms and Conditions
  • Privacy Policy

@2021 - All Right Reserved. Rantdriven

Rantdriven
  • News
  • Business
  • Gaming
  • Movie
  • Technology
  • More
    • About Us
    • Contact Us
    • Terms and Conditions
  • Write for us
@2021 - All Right Reserved. Rantdriven

Read alsox

endnote alternatives

April 14, 2022

netobjects fusions

December 18, 2021

contentstudio review

April 18, 2022